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How to Read a Peptide Certificate of Analysis

· Peptide Research, Quality & Testing

Start with identity, then purity

A useful Certificate of Analysis (COA) answers two questions first: is this the intended sequence or compound, and how much of the reported peak belongs to that species? Mass spectrometry supports identity. HPLC (or an equivalent chromatographic method) supports a purity percentage for the observed peak profile.

Those two numbers are related but not interchangeable. A high HPLC area percent does not, by itself, prove sequence. A matching mass does not, by itself, prove that related impurities are low. Review them together, against the lot number printed on the vial.

Fields worth checking on every COA

Common misreads

Purity is not the same as peptide content. A lot can be chromatographically clean and still differ in net peptide after salts or residual moisture. Appearance (“white lyophilized powder”) is a receiving check, not a potency assay. If a COA is missing a method, a date, or a lot that matches the label, ask for the file that belongs to that shipment before the material goes into a study series.

Biofusion Wellness publishes lot documentation on product pages where available and can reissue a COA against an order number.

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