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HPLC and Mass Spectrometry in Peptide Quality Review

· Quality & Testing

Two methods, two jobs

High-performance liquid chromatography separates components in a sample so a detector can estimate relative peak area. Mass spectrometry reports mass-to-charge information that supports molecular identity. Used together, they are the core of most research-peptide release packages.

Independent testing matters because it sits outside production. A third-party chromatogram is not a marketing graphic; it is a record that a named lot was evaluated under a stated method. Keep the PDF with the lot. If a later assay behaves unexpectedly, the first question is whether the vial in the freezer is the lot on the COA.

What “≥99%” usually means

Reported HPLC purity is typically an area-percent of the main peak under the conditions used. It is not a clinical specification, and it is not a guarantee that every possible impurity is visible at every wavelength. Treat it as a lot-release snapshot, then store and handle the material so that snapshot still describes what you pipette months later.

For method questions on a specific Biofusion Wellness batch, include the product name and lot in your email to info@biofusionwellness.com.

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